CD45RO+TILs: Cellular biomarkers for larynx squamous cell carcinoma outcome

ucjournals February 27, 2024

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Presented in 13th Emirates Pathology, Digital Pathology & Cancer Conference Holiday Inn Dubai, UAE & Virtual
Speaker Name: Dr. Maryam Mirtalebi
Research interest: Molecular pathology and cytogenetic
Global Journal of Pathology & Laboratory Medicine
Unified Citation Journals, Pathology 2023, ISSN 2754-0952
Keywords: Head and neck squamous cell carcinoma, Tu- mor infiltrating lymphocytes, Prognostic marker, CD45RO+ memory cells, Larynx squamous cell carcinoma

Biography: Maryam Mirtalebi has completed her AC/PC from Isfahan University of Medical Sciences. She has complet- ed her fellowship of molecular pathology and cytogenetic from Shiraz, University of Medical Sciences. She head pathology department of Pooyesh laboratory. She has published more than 5 abstracts in different journals.

Introduction:
The prognostic importance of Tumor-Infiltrating Lymphocytes (TILs) in the tumor microenvironment of various cancers is increasingly recognized. In the present study, we aimed to investigate the prognostic value of CD3+, CD4+, CD8+, and CD45RO + TILs and their relation to histopatholog- ical features in larynx squamous cell carcino- ma.
Methods:Formalin-Fixed and Paraffin-Embedded (FFPE) samples from 63 primary larynx squamous cell carcinoma pa- tients were immunostained for CD3, CD4, CD8, and CD45RO expression. Positive cells in micrographs from Invasive Margin (IM) and Tumor Center (CT) of tissue specimens counted by ImageJ software and their correlation with disease outcome were analyzed.
Results:The expression level of TILs subpopula- tions was associated with clinicopathological markers as well as Overall Survival (OS) and Disease-Free Survival (DFS). In mul- tivariate analysis, high frequency of CD45RO + cells in IM were confirmed as an independent prognostic marker for DFS (p = 0.007, HR = 4.968) and OS (p = 0.007, HR = 4.957). Simi-lar findings were observed in the multivariate analysis of the combined frequency of CD45RO+cells in IM and CT.
Conclusion:TILs are associated with patients clinicopatho- logical features. Also, our findings indicate that CD45RO + TILs are a valuable marker for risk prediction in larynx SCC and could predict patients’ outcomes.

References:
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Cancer, Immunodeficiency States, Immunophenotyping, Molecular Diagnostics & Proteomics, Evolutionary Medicine, Functional Identification & Biomarkers, Hematopoietic & other Malignancies, Anatomical Pathology, Clinical Pathology, Dermato Pathology, Forensic Pathology, Hemato Pathology, Histopathology, Molecular Pathology, Surgical Pathology, Histopathology, Chemical Pathology, Hematopathology, Histopathology, Cytopathology, Forensic Pathology, Dermatopathology, Clinical Biochemistry, Infection Control, Cytokines, Enzymology, Endocrinology, Cellular Lineage, Virology, Rheology, Toxicology, Neuropathology, Diagnostic Pathology, Mesothelial Proliferations, Transfusion medicine, Clinical microbiology, Cytogenetics, Molecular Genetics Pathology, Immunopathology, Veterinary Pathology, Anatomical Pathology, General Pathology