FROM ISOLATION TO IMPLEMENTATION: DEVELOPING EFFECTIVE PROBIOTIC FORMULATIONS FOR LIVESTOCK

Description:
Presented in: 15th World Probiotics and Gut Health Conference
Date: December 17-19, 2025
Venue: Novotel Al Barsha, Dubai, UAE
Name: Dr. Tahani Al-Surrayai

Al-Surrayai¹, H. Al-Khalaifah², T. Al-Onaizi³, M. Kishk4, H. Sultan⁵, H. Al-Seleem⁶, S. Al-Defilige⁷ and B, Almutairi⁸.

Introduction:
Probiotics have become a valuable approach for providing sustainable options to replace antibiotics for enhancing gut health, reduce the pathogenic load, improve nutrient digestion through enzymatic activity, and enhanced intestinal morphology. These advantageous microorganisms are key in maintaining the equilibrium of intestinal microbiota, which boosts immune function and lowers the risk of pathogenic infections. In cattle, probiotics contribute to rumen stability, improve fiber utilization, and enhance metabolic resilience, resulting in greater weight gain and higher milk productivity. Its effectiveness depends on selecting the appropriate probiotic strains, administering them in appropriate doses, and incorporating them into well-organized feeding and husbandry routines.

Aim: To evaluate the efficiency of using combined strains to serve as a probiotic product and find out the optimal isolates to the LAB combination and compatibility tests results. According to Tahani (2014) [1], the bacterial strains used were locally isolated, selected for their suitability under local, making them potentially more effective than imported probiotic products. Based on in vitro assessments of growth characteristics and safety for calves, the Lactobacillus strains were selected: Pediococcus pentosaceus, Lactobacillus fermentum, Lactobacillus rhamnousus, Lactobacillus plantarum, Lactobacillus brevis, and Lactobacillus casei. Compatibility among the six lactic acid bacterial (LAB) isolates was evaluated using a cross-streak assay following Kamat and Velho-Pereira (2011) [2].

Result: In developing a multi-strain probiotic product, it is critical that the LAB strains coexist without inhibiting one another (viability and metabolic activity). Cross-streak assays among the six isolates revealed a high degree of mutual tolerance: 88.9% of all pairwise interactions showed no growth interruption of the indicator strain, indicating that most combinations are compatible.

Keywords: Cattle. Compatibility, antagonistic

Fig, 1, Antimicrobial activity against Selected strains

Table 1. The compatibility test

References:

[1] Tahani Al Surrayai. Isolation, Characterization of Lactic Acid Bacteria for Development of Cattle Probiotics (Phase 1), 2014. https://pure.kfas.org.kw/en/projects/24f0d34f-8dd5-40fe-9443-d3830028e3bd. [2] Kamat, N.; Velho-Pereira, S. Antimicrobial Screening of Actinobacteria Using a Modified Cross-Streak Method. Indian J. Pharm. Sci. 2011, 73 (2), 223. https://doi.org/10.4103/0250-474X.91566.

                                       

BIOGRAPHY
Tahani Al-Surrayai has completed her B. SC in Biochemistry from Kuwait university and joined the Biotechnology program at KISR in 1995. During the past years, she developed excellent technical skills and has actively participated in several important research projects in the area of Environmental Biotechnology. Besides her depth of knowledge and skills in biochemistry and microbiology, she involved in the development of probiotic bacteria for use in livestock and poultry. She has over 28 publications that have been cited.

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